Journal: Endocrinology

Article Title: Adiponectin Exerts Neurotrophic Effects on Dendritic Arborization, Spinogenesis, and Neurogenesis of the Dentate Gyrus of Male Mice

doi: 10.1210/en.2015-2078

Figure Lengend Snippet: Antibody Table

Article Snippet: Next day, the sections were coincubated with the following Alexa Fluor-conjugated secondary antibodies for 4 hours: antirabbit Alexa Fluor 647 goat IgG, antimouse Alexa Fluor 594 goat IgG, and antirat Alexa Fluor 488 goal IgG (1:400; Life Technologies) ( ). table ft1 table-wrap mode="anchored" t5 Table 1. caption a7 Peptide/Protein Target Antigen Sequence (if Known) Name of Antibody Manufacturer, Catalog Number, and/or Name of Individual Providing the Antibody Species Raised in; Monoclonal or Polyclonal Dilution Used Ki67 Synthetic peptide conjugated to KLH derived from within residues 1200 − 1300 of Human Ki67 Anti-Ki67 antibody Abcam, Ab15580 Rabbit; polyclonal 1:1000 Doublecortin (DCX) epitope mapping at the C-terminus of Doublecortin of human origin Doublecortin antibody (C-18) Santa Cruz Biotechnology, Inc, sc-8066 Goat; polyclonal 1:200 BrdU Anti-BrdU antibody [BU1/75 (ICR1)] Accurate Chemical, YSRTMCA2060GA Rat; monoclonal 1:400 NeuN Anti-NeuN antibody, clone A60 Millipore, MAB377 Mouse; monoclonal 1:1000 GFAP Anti-glial fibrillary acidic protein (GFAP) antibody Millipore, AB5804 Rabbit; polyclonal 1:1000 Biotinylated antirabbit IgG Biotinylated goat antirabbit IgG antibody Vector Laboratories, BA-1000 Goat 1:400 Biotinylated antigoat IgG Biotinylated horse antigoat IgG antibody Vector Laboratories, BA-9500 Horse 1:400 Alexa Fluor 488 goat antirat IgG Goat antirat IgG (H + L) secondary antibody, Alexa Fluor 488 conjugate Invitrogen, {"type":"entrez-nucleotide","attrs":{"text":"A11006","term_id":"492389","term_text":"A11006"}} A11006 Goat 1:400 Alexa Fluor 546 goat antimouse IgG F(ab')2-goat antimouse IgG (H + L) secondary antibody, Alexa Fluor 546 conjugate Invitrogen, {"type":"entrez-nucleotide","attrs":{"text":"A11018","term_id":"492393","term_text":"A11018"}} A11018 Goat 1:400 Alexa Fluor 647 goat antirabbit IgG F(ab')2-goat antirabbit IgG (H + L) secondary antibody, Alexa Fluor 647 conjugate Invitrogen, {"type":"entrez-nucleotide","attrs":{"text":"A21246","term_id":"641368","term_text":"A21246"}} A21246 Goat 1:400 Open in a separate window Abbreviation: H+L, Heavy and light chain.

Techniques: Sequencing, Derivative Assay, Plasmid Preparation

Journal: Journal of the Formosan Medical Association = Taiwan yi zhi

Article Title: An efficient method for decellularization of the rat liver.

doi: 10.1016/j.jfma.2013.05.003

Figure Lengend Snippet: Figure 2 (AeH) Histologic comparison of normal liver (A,C,E,G) and decellularized liver matrix (B,D,F,H): hematoxylin and eosin staining (AeB); Masson trichrome staining (CeD); immunohistochemistry staining of fibronectin (EeF) and laminin (GeH). Western blot showed the expression of collagen I collagen II, fibronectin, and laminin, which was compatible with normal liver (I). (JeL) Extracellular matrix showed invisible cells in decellularized matrix (J), matrix retains an intact tiny lobular structure (K) and main vasculature (L). Magnification: AeF 100, GeH 200, J and K 3000, and L 1200.

Article Snippet: The samples were collected from decellularized bioscaffolds and fresh liver and embedded, sectioned, dehydrated with gradient ethanol, and then incubated with 1:100 diluted primary rabbit antirat fibronectin or rabbit antirat laminin antibodies (Boster Biological Technology Ltd., Wuhan, China).

Techniques: Comparison, Staining, Immunohistochemistry, Western Blot, Expressing